Can we consign “The Edge Effect” to history?
We can waste about 40% of multi-well plates to avoid biasing the relative potency (RP) due to the “edge effect”. Quantics recently used a Latin square design to support a batch release assay designed to eliminate variation across the whole assay plate.
This design allowed row and column effects to be identified and correctly accounted for in the estimation of reportable values, avoiding assay bias. The assay data clearly demonstrated that row and column effects are sometimes seen and, most importantly, can be managed.
Key Takeaways
- Edge effects can waste up to 40% of multi-well plate capacity if not properly addressed.
- Latin square designs allow row and column effects to be identified and adjusted for.
- Simulation studies show substantial bias reduction when these techniques are applied.
In this presentation we describe the Latin square and explain how similar, more practical designs can be used in a typical multi-well plate assay to overcome these issues and maximise assay efficiency. We also show the results of a simulation study demonstrating the extent of estimation bias that can be avoided using this technique.
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